RESUMO
Introduction: currently, the most relevant applications of perinatal derivatives are in wound healing, for example, the use of amniotic membrane (AM) as a biomaterials source for use in different healing processes. The anastomotic healing compromised by antimetabolic drugs, such as 5-fluorouracil (5-FU), is a potential target of AM. Objective: To evaluate the healing effects of the AM use in rats treated with 5-FU at a dose of 20 mg/kg on the 7th postoperative day, regarding the parameters percentage of type I collagen (mature), cell viability, microvascular density and granulation tissue formation. Method: Thirty-two Wistar rats were submitted to colotomy and colorraphy, separated in 4 groups with 8t rats each and received, by 7 days, different treatments intraperitoneally, once daily from the operation day until sacrifice: 1- saline solution (C), 2- 20 mg/kg 5-FU, 3- 20 mg/kg 5-FU and 4- MA, and MA. Results: Tthe treatment with 5-FU (20 mg/kg 7 days) induced adverse effects in the anastomotic healing process evidenced by the decrease of type I collagen (mature), cell viability, percentage microvascular density, granulation tissue formation (leukocyte-fibrin crust and angio-fibroblastic proliferation). The use of AM, under these conditions, induced an improvement in type I collagen (mature) percentage. Conclusion: The treatment with 5-FU induced adverse effects on the healing anastomotic process, and the use of AM, under these conditions, induced improvement in type I (mature) collagen percentage.
Introdução: Atualmente emerge em nosso meio aplicações mais relevantes dos derivados perinatais, por exemplo, a membrana amniótica (MA), como fonte de biomateriais para emprego em diferentes processos cicatriciais. O comprometimento anastomótico por drogas antimetabólicas como o 5-fluorouracil (5-FU) é um potencial alvo da MA. Objetivo: Avaliar os efeitos cicatriciais do uso da MA em ratos tratados com 5-FU na dose de 20 mg/kg ao 7º. dia de evolução pós-operatória quanto aos parâmetros porcentagem de colágeno tipo I (maduro), viabilidade celular, densidade microvascular e formação do tecido de granulação. Método: Utilizaram-se 32 ratos da linhagem Wistar submetidos à colotomia e colorrafia separados em 4 grupos com 8 e receberam diferentes tratamentos diariamente por via intraperitoneal até o dia do sacrifício: Solução fisiológica (C), 20 mg/kg 5-FU, 20 mg/kg 5-FU e MA, MA. Resultados: O tratamento com 20 mg/kg de 5-FU, ao 7º. dia pós-operatório induziu efeitos adversos no processo cicatricial anastomótico evidenciados pela diminuição da porcentagem de colágeno tipo I (maduro), da viabilidade celular, da densidade microvascular, da formação de crosta fibrinoleucocitária e da proliferação angiofibroblástica; o uso da MA nestas condições induziu melhora da porcentagem de colágeno tipo I (maduro). Conclusão: O tratamento com 20 mg/kg de 5-FU, ao 7º. dia pós-operatório induziu efeitos adversos no processo cicatricial anastomótico e o uso da MA nestas condições induziu melhora da porcentagem de colágeno tipo I (maduro).
RESUMO
PURPOSE: To compare ileal anastomoses in the immediate postoperative healing period after meloxicam use. METHODS: Forty two male Wistar rats were randomly divided into two groups of 21, COX and control group. To COX meloxicam in combination with morphine was given in 3 days period. Control group received only morphine during the same period. Each group was divided into three sub-groups of 7, which were euthanized at 5, 10, and 21 days postoperatively. Comparison was based in histological evaluation of collagen type I and III using sirius red, immunohistochemical through vascular endothelial growth factor and matrix metalloproteinase-9. RESULTS: Healing process in scheduled periods did not show significant differences (p>0.05) between the COX and control groups during any of the periods. CONCLUSION: The use of meloxicam in the postoperative period following ileal anastomosis did not affect healing.
Assuntos
Anti-Inflamatórios não Esteroides/farmacologia , Inibidores de Ciclo-Oxigenase/farmacologia , Íleo/cirurgia , Tiazinas/farmacologia , Tiazóis/farmacologia , Cicatrização/efeitos dos fármacos , Anastomose Cirúrgica , Animais , Colágeno Tipo I/metabolismo , Colágeno Tipo III/metabolismo , Íleo/irrigação sanguínea , Masculino , Metaloproteinase 9 da Matriz/efeitos dos fármacos , Metaloproteinase 9 da Matriz/metabolismo , Meloxicam , Modelos Animais , Neovascularização Fisiológica/efeitos dos fármacos , Período Pós-Operatório , Distribuição Aleatória , Ratos Wistar , Receptores de Fatores de Crescimento do Endotélio Vascular/efeitos dos fármacos , Receptores de Fatores de Crescimento do Endotélio Vascular/metabolismo , Fatores de TempoRESUMO
ABSTRACT PURPOSE : To compare ileal anastomoses in the immediate postoperative healing period after meloxicam use. METHODS: Forty two male Wistar rats were randomly divided into two groups of 21, COX and control group. To COX meloxicam in combination with morphine was given in 3 days period. Control group received only morphine during the same period. Each group was divided into three sub-groups of 7, which were euthanized at 5, 10, and 21 days postoperatively. Comparison was based in histological evaluation of collagen type I and III using sirius red, immunohistochemical through vascular endothelial growth factor and matrix metalloproteinase-9. RESULTS: Healing process in scheduled periods did not show significant differences (p>0.05) between the COX and control groups during any of the periods. CONCLUSION: The use of meloxicam in the postoperative period following ileal anastomosis did not affect healing.
